Parisa Yazdanshenas; Raana Jahanbin; Abdoullah Mohammadi Sangcheshmeh; Mehdi Aminafshar; Hossein Vaseghi Dodaran; Hamid Varnaseri; Mohammad Chamani; Mohammad Hassan Nazaran
Volume 18, Issue 1 , April 2016, , Pages 173-181
Abstract
This study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. Ejaculates were collected from four Holstein bulls, twice a week during four weeks. Semen samples diluted with Bioxcell extender containing ...
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This study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. Ejaculates were collected from four Holstein bulls, twice a week during four weeks. Semen samples diluted with Bioxcell extender containing zero (Control), 10-6, 10-5, 10-4, 10-3 and 10-2 M of zinc nano-complex. The samples were then freezed-thawed after 72 hours. Motility of sperm was analyzed using computer assisted sperm analysis. The highest and lowest concentrations of zinc nano-complex along with control group were analyzed for the DNA fragmentation, malondialdehyde (MDA) concentration, mitochondrial activity, and pregnancy outcomes. The proportion of the total and progressive motile spermatozoa and the percentage of spermatozoa with fragmented DNA were not different among the groups. However, the groups containing 10-6 and 10-2 M zinc nano-complex had a lower level of MDA concentration and higher activity of mitochondria. Moreover, the pregnancy outcome was not affected by the treatment with zinc nano-complex. In conclusion, our data revealed that supplementation of bull semen Bioxcell extender with zinc nano-complex can decrease the level of MDA and improve mitochondrial activity, although such an improvement was not reflected in results of fertility rates.
Raana Jahanbin; Parisa Yazdanshenas; Mehdi Amin Afshar; Abdollah Mohammadi Sangcheshmeh; Hamid Varnaseri; Mohammad Chamani; Mohammad Hasan Nazaran; Mohammad Reza Bakhtiyarizadeh
Volume 17, Issue 2 , October 2015, , Pages 371-380
Abstract
The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, ...
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The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc Nano-complex and frozen. After thawing, sperm motility and motion parameters, plasma membrane integrity, abnormal morphology, plasma membrane functionality and mitochondrial activity were determined. The proportion of the total and progressive motile sperm, the plasma membrane integrity and proportion of the spermatozoa with abnormal morphology was not different among groups. Zinc Nano-complex groups represented a higher plasma membrane functionality than that of control group. Moreover, our flowcytometric data suggested that spermatozoa in the groups of zinc Nano-complex possessed higher mitochondrial activity as compared with the control group. Mitochondrial activity in 10-2 was higher than the 10-6 group. In conclusion, supplementation of zn Nano-complex, can improve the plasma membrane functionality and mitochondrial activity of bull spermatozoa in a dose dependent manner without any deleterious effect on motility parameters.